Molecular Detection of Chlamydia Trachomatis in Patients with Pelvic Inflammatory Disease Visiting a Tertiary Care Hospital in Pakistan

Objective: To detect the frequency of Chlamydia trachomatis in urine samples women with Pelvic Inflammatory Disease visiting a tertiary care hospital using Polymerase Chain Reaction assay and find an association between different risk factors.
 Study Design: Cross-sectional study.
 Place Duration Study: Department Microbiology, Army Medical College/National University Sciences Rawalpindi Pakistan, from Mar 2018 to Jan 2019.
 Methodology: Over eleven months, 60 diagnosed married females pelvic inflammatory disease ages 14-49 years were included study. DNA was extracted manually commercially available kit. It detected by performing real-time forward primer (5’-CATGAAAACTCGTTCCGAAATAGAA-3’) reverse (5’-TCAGAGCTTTACCTAACAACGCATA-3’) sequence mentioned above for amplification target sequences, trachomatis.
 Results: Out sixty cases this study, 12 (20%), 48 (80%) negative. So, our study population estimated at 20%. In addition, age, socioeconomic status, education no sexual partners all factors evaluated their role acquiring infection.
 Conclusion: Females having infection other sexually transmitted infections are increased developing due presence asymptomatic nature disease.